This paper tries to recognize the substantiation through high performance liquid chromatography (HPLC) on the Dipyridamole and Aspirin, the separation was achieved on aX-Terra RP-18 5µm, 50 mm x 4.6 mm using a mobile phase consisting of 0.1% Ortho phosphoric acid and Acetonitrile in the ratio of 75:25 at a flow rate of 1.0 ml per minute. The detection was made at 227nm. The retention time of Aspirin and Dipyridamole were 1.5 and 2.8 minutes respectively. The proposed method was validated as per the ICH and USP guidelines. The method was found linear over the range of 4-80 µg per ml for Dipyridamole and 0.5-10 µg per ml for Aspirin.The experimental are peak purity and thresholdAspirin and Dipyridamolepeakswere met acceptance criteria.
