In the present study protease enzyme was purified from sea weed, Gracilaria fergusonii by gel filtration chromatography using Sephadex G-75 and their molecular mass was determined on SDS-PAGE. The protease enzyme activity was initially verified by protease assay. The enzyme was highly active in 2.0 % of substrate concentration and CaCl2 necessary to their enzyme activity. The optimum temperature at 50ºC and pH at 8.
