Soil microorganisms play an important role in soil fertility and promoting plant health. Their populations play an important role in plant growth and regulations. Soil harbors maximum biodiversity. This study was undertaken to investigate the diversity among fungal flora in the soil samples from villages in and around Jhansi. Soil samples were collected from10 different agriculture fields of pulses. A total of 14 fungal species belonging to 6 genera, were isolated on potato dextrose agar media (PDA), by using soil dilution technique. Identification and characterization of the mycoflora was done with the help of Introductory Mycology by Alexopolus et al. (2017) and was confirmed from NCFT, New Delhi. The overall dominant genera were Trichoderma, Aspergillus, Fusarium and Penicillium.