Fuel belongs to the non-renewable energy resources, which is depleting in rapid amount. Thus throughout world every country is investing huge time and money. There is always a thirst to identify an alternative source for fuel production. In this current research an attempt is taken to produce cellulytic bioethanol production by Saccahromyces cerevisiae using Agave plant as the source of the substrate for bioethanol production. The bioethanol production was confirmed by the reaction with potassium dichromate. The bioprocessing of the ethanol production was carried out as static and shaking conditions. The bioethanol production was found to be maximum in shaking conditions. Further research must be carried out to have a complete understanding about the bioprocessing involved in the the bioethanol production at commercial level.
