Community-acquired infections, particularly respiratory tract and urinary tract infections are caused by Escherichia coli represents a significant burden for most health care systems in the globe. The excessive use of commercial antibiotics leads to raising resistance in microbes and cause severe side effects. There is an urgent need to require alternate antibiotics to overcome this problem. The current study was deals with the isolation and identification of multi-drug resistant E. coli from clinical samples. In addition, we perform green synthesis of silver nanoparticles using Clausena dentata leaf extract and its antibacterial potential. The biosynthesized AgNPs were characterized by SEM-EDAX, XRD, UV–Vis and FT-IR spectroscopy techniques. UV-Vis spectroscopy of AgNPs showed maximum absorption at 430-460 nm. XRD and SEM analysis revealed that AgNPs are face-centered, a cubic structure being spherical in shape with an average particle size of 40-50 nm. The antibacterial activity result highlights biosynthesized silver nanoparticles reported more potent activity against multi-drug resistant E. coli compared to control.
